TABLE 4.
Function/description | Response | Symbol | Fold change in gene expression on day:
|
||||
---|---|---|---|---|---|---|---|
1 | 2 | 7 | 14 | 28 | |||
Antiviral response | |||||||
Interferon-stimulated gene 20 kDa | Marked | ISG20 | 3.21 | 2.49 | 2.21 | 2.07 | 2.16 |
Interferon-stimulated gene 20 kDa | Poor | ISG20 | 2.75 | 2.11 | 1.65 | 1.50 | 1.57 |
2′5′-Oligoadenylate synthetase 1 (40/46 kDa) | Marked | OAS1 | 7.03 | 6.78 | 4.18 | 3.80 | 4.29 |
2′5′-Oligoadenylate synthetase 1 (40/46 kDa) | Poor | OAS1 | 4.94 | 4.86 | 2.82 | 2.98 | 3.01 |
2′5′-Oligoadenylate synthetase 2 (69/71 kDa) | Marked | OAS2 | 5.10 | 4.91 | 3.88 | 4.04 | 4.44 |
2′5′-Oligoadenylate synthetase 2 (69/71 kDa) | Poor | OAS2 | 3.67 | 3.55 | 2.57 | 2.63 | 2.77 |
2′5′-Oligoadenylate synthetase-like | Marked | OASL | 7.83 | 6.72 | 3.57 | 4.09 | 4.16 |
2′5′-Oligoadenylate synthetase-like | Poor | OASL | 5.56 | 4.94 | 2.64 | 2.63 | 2.99 |
Viperin, cig5 | Marked | RSAD2 | 18.64 | 17.94 | 10.3 | 14.28 | 15.58 |
Viperin, cig 5 | Poor | RSAD2 | 12.11 | 12.1 | 6.70 | 7.13 | 7.66 |
Apoptosis | |||||||
XIAP associated factor 1 | Marked | HSXIAPAF1 | 3.48 | 4.00 | 3.42 | 3.74 | 3.52 |
XIAP associated factor 1 | Poor | HSXIAPAF1 | 2.87 | 2.99 | 2.36 | 2.41 | 2.56 |
TRAIL | Marked | TNFSF10 | 5.4 | 4.33 | 2.31 | 2.16 | 2.42 |
TRAIL | Poor | TNFSF10 | 3.28 | 2.99 | 1.56 | 1.59 | 1.67 |
Cell proliferation | |||||||
Lysosomal-associated membrane protein 3 | Marked | LAMP3 | 11.52 | 6.61 | 5.5 | 5.13 | 6.19 |
Lysosomal-associated membrane protein 3 | Poor | LAMP3 | 7.18 | 4.57 | 2.78 | 2.69 | 3.45 |
Chemokines | |||||||
Chemokine (C-C motif) ligand 2 | Marked | CCL2 | 58 | 41.07 | 16.24 | 23.59 | 15.65 |
Chemokine (C-C motif) ligand 2 | Poor | CCL2 | 52.43 | 27.33 | 7.07 | 75.62 | 10.38 |
Chemokine (C-C motif) receptor 1 | Marked | CCR1 | 4.22 | 3.88 | 2.21 | 2.86 | 2.82 |
Chemokine (C-C motif) receptor 1 | Poor | CCR1 | 2.84 | 2.86 | 1.78 | 2.08 | 1.84 |
Chemokine (C-C motif) ligand 8 | Marked | CCL8 | 11.18 | 5.28 | 2.01 | 2.47 | 2.85 |
Chemokine (C-C motif) ligand 8 | Poor | CCL8 | 7.04 | 2.70 | 1.40 | 1.57 | 1.56 |
Chemokine (C-X-C motif) ligand 11 | Marked | CXCL11 | 5.09 | 2.22 | −1.01 | 1.00 | −1.07 |
Chemokine (C-X-C motif) ligand 11 | Poor | CXCL11 | 4.83 | 2.66 | 3.66 | 1.33 | 1.22 |
Chemokine (C-X-C motif) ligand 10 | Marked | CXCL10 | 24.42 | 11.88 | 1.79 | 1.93 | 2.00 |
Chemokine (C-X-C motif) ligand 10 | Poor | CXCL10 | 19.95 | 8.94 | 1.51 | 1.47 | 1.56 |
Complement pathway | |||||||
Complement component 3a receptor 1 | Marked | C3AR1 | 3.62 | 3.28 | 1.76 | 2.22 | 1.92 |
Complement component 3a receptor 1 | Poor | C3AR1 | 2.38 | 2.32 | 1.35 | 1.49 | 1.33 |
Serine (or cysteine) proteinase inhibitor | Marked | SERPING1 | 11.93 | 7.09 | 2.19 | 2.57 | 2.43 |
Serine (or cysteine) proteinase inhibitor | Poor | SERPING1 | 8.88 | 5.65 | 1.94 | 2.29 | 2.24 |
Endonuclease | |||||||
Liver RNase (neurotoxin) | Marked | RNAse2 | 2.85 | 2.70 | 2.10 | 2.71 | 2.24 |
Liver RNase (neurotoxin) | Poor | RNAse2 | 2.19 | 2.13 | 1.74 | 1.64 | 1.82 |
Helicases | |||||||
Hypothetical protein FLJ20035 | Marked | FLJ20035 | 4.56 | 4.38 | 4.10 | 4.06 | 4.27 |
Hypothetical protein FLJ20035 | Poor | FLJ20035 | 3.36 | 2.85 | 2.42 | 2.16 | 2.66 |
Interferon induced with helicase C domain 1 | Marked | IFIH1 | 5.59 | 4.99 | 3.52 | 3.70 | 4.21 |
Interferon induced with helicase C domain 1 | Poor | IFIH1 | 3.59 | 3.25 | 2.24 | 2.20 | 2.48 |
Immune response | |||||||
Alpha interferon-inducible protein (clone IFI-15K) | Marked | G1P2 | 10.51 | 9.76 | 6.53 | 7.72 | 8.35 |
Alpha interferon-inducible protein (clone IFI-15K) | Poor | G1P2 | 7.43 | 6.93 | 3.89 | 4.24 | 4.75 |
Alpha interferon-inducible protein (clone IFI-6-16) | Marked | G1P3 | 4.11 | 4.09 | 3.02 | 3.22 | 3.07 |
Alpha interferon-inducible protein (clone IFI-6-16) | Poor | G1P3 | 3.39 | 2.67 | 1.73 | 1.99 | 2.29 |
Alpha interferon-inducible protein 27 | Marked | IFI27 | 27.98 | 41.54 | 61.26 | 100.47 | 121.84 |
Alpha interferon-inducible protein 27 | Poor | IFI27 | 21.45 | 35.28 | 52.67 | 69.51 | 89.4 |
Interferon-induced protein 35 | Marked | IFI35 | 4.75 | 4.18 | 2.77 | 2.58 | 2.71 |
Interferon-induced protein 35 | Poor | IFI35 | 3.56 | 3.00 | 1.82 | 1.87 | 1.95 |
Interferon-induced protein 44 | Marked | IFI44 | 6.62 | 6.31 | 6.03 | 6.59 | 6.77 |
Interferon-induced protein 44 | Poor | IFI44 | 4.29 | 4.12 | 3.41 | 3.36 | 4.09 |
Interferon-induced protein 44 | Marked | IFI44L | 9.09 | 9.36 | 9.13 | 10.68 | 10.67 |
Interferon-induced protein 44 | Poor | IFI44L | 5.91 | 5.93 | 5.02 | 4.95 | 6.06 |
Interferon-induced protein with tetratricopeptide repeats 1 | Marked | IFIT1 | 17.57 | 17.1 | 10.72 | 12.27 | 13.55 |
Interferon-induced protein with tetratricopeptide repeats 1 | Poor | IFIT1 | 9.23 | 9.23 | 5.79 | 5.27 | 6.31 |
Interferon-induced protein with tetratricopeptide repeats 3 | Marked | IFIT3 | 11.95 | 10.3 | 6.38 | 7.53 | 8.47 |
Interferon-induced protein with tetratricopeptide repeats 3 | Poor | IFIT3 | 7.42 | 6.73 | 3.56 | 3.74 | 4.23 |
Interferon-induced protein with tetratricopeptide repeats 5 | Marked | IFIT5 | 3.32 | 3.19 | 2.63 | 2.72 | 2.94 |
Interferon-induced protein with tetratricopeptide repeats 5 | Poor | IFIT5 | 2.49 | 2.30 | 2.05 | 2.02 | 2.00 |
Interferon-induced transmembrane protein 1 (9-27) | Marked | IFITM1 | 1.86 | 1.76 | 1.67 | 1.71 | 1.95 |
Interferon induced transmembrane protein 1 (9-27) | Poor | IFITM1 | 1.71 | 1.63 | 1.42 | 1.41 | 1.62 |
Interferon-induced transmembrane protein 3 (1-8U) | Marked | IFITM3 | 2.71 | 2.45 | 1.89 | 2.00 | 1.91 |
Interferon-induced transmembrane protein 3 (1-8U) | Poor | IFITM3 | 2.28 | 2.20 | 1.60 | 1.65 | 1.82 |
IL-1 receptor antagonist | Marked | IL1RN | 5.83 | 4.12 | 1.43 | 1.99 | 1.98 |
IL-1 receptor antagonist | Poor | IL1RN | 4.21 | 2.93 | 1.44 | 2.05 | 1.75 |
Myxovirus (influenza virus) resistance 1 | Marked | MX1 | 6.51 | 5.80 | 4.95 | 5.57 | 5.39 |
Myxovirus (influenza virus) resistance 1 | Poor | MX1 | 4.29 | 3.90 | 2.83 | 2.87 | 3.26 |
Myxovirus (influenza virus) resistance 2 | Marked | MX2 | 4.39 | 3.89 | 3.07 | 3.28 | 3.30 |
Myxovirus (influenza virus) resistance 2 | Poor | MX2 | 3.46 | 2.94 | 2.04 | 2.12 | 2.49 |
Inflammatory response | |||||||
Heparanase | Marked | HPSE | 4.16 | 2.45 | 1.73 | 2.05 | 1.80 |
Heparanase | Poor | HPSE | 2.97 | 1.92 | 1.39 | 1.50 | 1.59 |
Sialoadhesin | Marked | SN | 44.54 | 39.65 | 23.14 | 33.58 | 26.4 |
Sialoadhesin | Poor | SN | 18.17 | 16.45 | 9.37 | 11.92 | 12.41 |
JAK-STAT cascade | |||||||
N-myc (and STAT) interactor | Marked | NMI | 3.07 | 2.68 | 1.91 | 1.93 | 2.08 |
N-myc (and STAT) interactor | Poor | NMI | 2.19 | 2.01 | 1.50 | 1.46 | 1.59 |
Signal transducer and activator of transcription 1 (91 kDa) | Marked | STAT1 | 2.38 | 2.05 | 1.88 | 1.85 | 2.09 |
Signal transducer and activator of transcription 1 (91 kDa) | Poor | STAT1 | 1.98 | 1.77 | 1.46 | 1.37 | 1.61 |
Phospholipid scrambling | |||||||
Phospholipid scramblase 1 | Marked | PLSCR1 | 4.61 | 4.22 | 2.92 | 3.32 | 3.50 |
Phospholipid scramblase 1 | Poor | PLSCR1 | 3.24 | 2.97 | 2.17 | 2.01 | 2.50 |
RNA editing | |||||||
Apolipoprotein B mRNA editing enzyme | Marked | APOBEC3A | 6.03 | 4.66 | 2.63 | 2.93 | 2.94 |
Apolipoprotein B mRNA editing enzyme | Poor | APOBEC3A | 4.29 | 3.53 | 2.08 | 2.15 | 2.22 |
Signal transduction | |||||||
Membrane-spanning 4 domains, subfamily A, member 4 | Marked | MS4A4A | 7.00 | 7.36 | 2.35 | 2.89 | 2.48 |
Membrane-spanning 4 domains, subfamily A, member 4 | Poor | MS4A4A | 3.68 | 4.39 | 1.61 | 1.71 | 1.89 |
Toll pathway | |||||||
Toll-like receptor 7 | Marked | TLR7 | 7.17 | 5.12 | 2.90 | 3.19 | 2.79 |
Toll-like receptor 7 | Poor | TLR7 | 3.93 | 3.07 | 1.70 | 1.66 | 1.80 |
Transcription factor/repressor | |||||||
CD38 antigen (p45) | Marked | CD38 | 2.61 | 2.15 | 1.89 | 1.86 | 1.90 |
CD38 antigen (p45) | Poor | CD38 | 2.43 | 2.03 | 1.64 | 1.46 | 1.56 |
Homeobox (expressed in ES cells) 1 | Marked | HESX1 | 23.01 | 17.4 | 9.44 | 10.46 | 9.53 |
Homeobox (expressed in ES cells) 1 | Poor | HESX1 | 17.56 | 12.78 | 9.01 | 5.74 | 6.23 |
Gamma interferon-inducible protein 16 | Marked | IFI16 | 2.28 | 2.05 | 1.94 | 1.80 | 1.80 |
Gamma interferon-inducible protein 16 | Poor | IFI16 | 1.84 | 1.87 | 1.55 | 1.45 | 1.51 |
Interferon regulatory factor 7 | Marked | IRF7 | 4.56 | 4.25 | 3.12 | 3.20 | 3.37 |
Interferon regulatory factor 7 | Poor | IRF7 | 3.34 | 3.11 | 2.04 | 2.19 | 2.33 |
Lectin, galactoside-binding, soluble, 9 (galectin 9) | Marked | LGALS9 | 3.04 | 2.86 | 1.86 | 1.81 | 2.07 |
Lectin, galactoside-binding, soluble, 9 (galectin 9) | Poor | LGALS9 | 2.30 | 2.07 | 1.32 | 1.61 | 1.65 |
Lymphocyte antigen 6 complex, locus E | Marked | LY6E | 4.59 | 4.93 | 3.68 | 3.31 | 3.85 |
Lymphocyte antigen 6 complex, locus E | Poor | LY6E | 3.71 | 3.71 | 2.25 | 2.95 | 2.86 |
Nuclear antigen Sp100 | Marked | SP100 | 2.04 | 1.90 | 1.89 | 1.82 | 1.75 |
Nuclear antigen Sp100 | Poor | SP100 | 1.78 | 1.78 | 1.51 | 1.37 | 1.43 |
SP110 nuclear body protein | Marked | SP110 | 1.98 | 2.03 | 1.76 | 1.80 | 1.85 |
SP110 nuclear body protein | Poor | SP110 | 1.64 | 1.64 | 1.46 | 1.46 | 1.53 |
T-box 3 (ulnar mammary syndrome) | Marked | TBX3 | 7.36 | 6.13 | 4.26 | 4.42 | 4.42 |
T-box 3 (ulnar mammary syndrome) | Poor | TBX3 | 4.91 | 4.02 | 2.47 | 2.35 | 2.6 |
Ubiquitin pathway | |||||||
Hect domain and RLD 5 | Marked | HERC5 | 8.64 | 7.50 | 4.64 | 5.06 | 5.46 |
Hect domain and RLD 5 | Poor | HERC5 | 5.92 | 5.09 | 2.88 | 2.85 | 3.18 |
Hect domain and RLD 6 | Marked | HERC6 | 5.61 | 5.46 | 5.84 | 5.54 | 5.61 |
Hect domain and RLD 6 | Poor | HERC6 | 4.19 | 4.07 | 3.59 | 3.35 | 3.76 |
Promyelocytic leukemia | Marked | PML | 3.68 | 2.89 | 1.80 | 1.93 | 1.91 |
Promyelocytic leukemia | Poor | PML | 3.07 | 2.26 | 1.49 | 1.60 | 1.60 |
Ubiquitin-conjugating enzyme E2L 6 | Marked | UBE2L6 | 2.97 | 2.52 | 1.76 | 1.81 | 1.82 |
Ubiquitin-conjugating enzyme E2L 6 | Poor | UBE2L6 | 2.54 | 2.19 | 1.42 | 1.45 | 1.53 |
Ubiquitin-specific protease 18 | Marked | USP18 | 12.53 | 10.06 | 7.10 | 8.66 | 9.96 |
Ubiquitin-specific protease 18 | Poor | USP18 | 6.89 | 5.80 | 4.00 | 3.95 | 4.89 |
Unknown function | |||||||
28-kDa interferon-responsive protein | Marked | IFRG28 | 4.68 | 4.87 | 3.38 | 3.52 | 3.76 |
28-kDa interferon-responsive protein | Poor | IFRG28 | 3.43 | 3.09 | 2.16 | 2.10 | 2.11 |
Changes in gene expression for marked responders and poor responders at days 1, 2, 7, 14, and 28 after initiation of treatment. Data were derived from Table S1 in the supplemental material. Numbers in boldface represent values below the cutoff of a 1.5-fold change or a P value of >0.001.