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. 2007 Jan 10;81(7):3162–3169. doi: 10.1128/JVI.02356-06

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Copyright © 2007, American Society for Microbiology

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FIG. 1. — Expression of SR-BII in CHO cells confers sE2 binding. (A) Anti-SR-BI serum reactivity for CHO (closed symbols) and CHO-SR-BI (open symbols). (B) sE2 reactivity for CHO (closed symbols) and CHO-SR-BI (open symbols). Bound E2 antigen was detected with rat anti-E2 monoclonal antibody 9/75 and Alexa Fluor 488 anti-rat Ig. The data are expressed as the mean fluorescence intensity (MFI). CHO cells were transduced with TRIP lentiviral vectors expressing human SR-BI or SR-BII and assessed for their reactivities with anti-SR-BI (1:400 dilution) (C) and sE2 (30 μg/ml) (D). Each panel displays parental CHO (filled), CHO-SR-BI (solid line), and CHO-SR-BII (dashed line).