FIG. 5.

Loss of STAT2 protein in RSV-infected HEp-2 cells is rescued with a siRNA against Rbx1 or Cul2. HEp-2 cells were transfected with a siRNA empty vector (Ev) or a siRNA directed against Cul2 (Cul2-1 and Cul2-2), Cul5 (Cul5-1 and Cul5-2), Rbx1 (Rbx1-1 and Rbx1-2), or Rbx2 (Rbx2-1 and Rbx2-2). A Lipofectamine transfection control was also included (Lc). Cells were then either mock infected or infected with RSV at an MOI of 15. Whole-cell lysates were analyzed for STAT2 (upper panels), STAT1 (middle panels), or γ- tubulin (lower panels) expression.