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. 2007 Jan 3;81(7):3109–3123. doi: 10.1128/JVI.02124-06

FIG. 9.

FIG. 9.

Expression of pUL38 in trans blocks apoptosis induced by the mutant adenovirus dl337. (A) HF-GFP or HF-UL38 cells were infected with dl337. The morphology of infected cells was examined under a phase-contrast microscope (top panel, multiplicity of 3 PFU/cell), and the rate of cell death in dl337-infected cells was quantitated by labeling surviving cells with DAPI and counting DAPI-positive cells (middle panel, 48 h postinfection; bottom panel, multiplicity of 1 PFU/cell). Six independent fields for each sample were counted, and the number of cells in each dl337-infected HF-GFP or HF-UL38 sample was plotted as a percentage of that in a mock-infected sample. (B) HeLa cell lines stably expressing either the empty vector (HeLa-dsRed) or pUL38 (HeLa-UL38) were infected with dl337, the morphology of infected cells was examined under a phase-contrast microscope (top panel, multiplicity of 3 PFU/cell, 40 h postinfection), and the rate of cell death in dl337-infected cells was quantitated as described for panel A (middle panel, 40 h postinfection; bottom panel, multiplicity of 1 PFU/cell). Two independently isolated HeLa-UL38 cell lines (HeLa-UL38-1 and HeLa-UL38-3) were examined.