FIG. 2.
Southern blots of transient DNA replication supported by UL42 mutant. The transient DNA replication of the oriS-containing plasmid (pHOS9.2) supported by the UL42 mutants was performed as described in Materials and Methods. Aliquots of purified DNA samples were digested with either EcoRII alone or with EcoRII plus DpnII, fractionated on a 0.8% agarose gel, and hybridized to a probe corresponding to the ColE1 sequence. (A) Results shown from the wild-type UL42 control (lanes 1 and 2) and the mutation R279A (lanes 3 and 4). (B) Results are shown of no UL42 DNA (lanes 1 and 2) and those of each mutant as well as the linearized pHOS1 (lane 11). The positions of linearized pHOS9.2 (bottom arrows) and pHC plasmids (top arrows) are indicated.