FIG. 3.
Single-cycle growth assay of UL42 recombinants. Vero cells (1 × 105) were inoculated with UL42 recombinants at an MOI of 3. Infected cells were harvested at 2, 5, 8, 12, 24, and 36 h postinfection and titrated with Vero cells to determine the yield of progeny viruses. Since recombinant A and B of each mutant exhibited similar growth kinetics, only results of recombinant A are shown to clearly demonstrate the difference of virus yields.