FIG. 4.
Viral DNA synthesized by UL42 recombinants. Vero cells (1 × 105) were inoculated with UL42 recombinants at an MOI of 3. Infected cells were harvested at different times postinfection. DNA was isolated from aliquots of infected cells, including both infected cells and medium containing cell-free virus. Purified DNA was serially diluted and quantified by real-time PCR as described in Materials and Methods to determine the relative copy number of viral DNA. Since recombinant A and B of each mutant replicated similar amounts of DNA, only results of recombinant A are shown to clearly demonstrate different amounts of DNA synthesized by these recombinants.