FIG. 7.
Association of Us11 with OAS in a nuclease-sensitive complex. Cells (293) were transfected with a Us11 expression plasmid (pGFP-Us11) in the presence and absence of an OAS expression plasmid (pFLAG-OAS). Twenty-four hours posttransfection, soluble cell-free lysates were prepared and immunoprecipitated with anti-FLAG monoclonal antibody (IP: FLAG). Immune complexes were fractionated by SDS-PAGE and analyzed by immunoblotting with anti-GFP (IB: GFP) or anti-FLAG (IB: FLAG). The bottom panel displays the overall abundance of the GFP-Us11 antigen in the starting lysates. Lysates treated with a nuclease cocktail (RNase A, T1, micrococcal nuclease) that degrades both single- and double-stranded RNA are designated by a + beneath the bottom panel.