FIG. 6.

LBS2 is dispensable for replication. (A) An empty vector and pBSpuro containing the MRE (RE-LBS1/2) with its deletion mutants (RE-LBS1, LBS1, and RE) were transfected into 293 cells along with the LANA expression vector. (B) Expression of LANA was detected by Western blotting (WB) with an anti-myc antibody. (C) DNAs extracted from these cells by the Hirt procedure 96 h posttransfection were digested with BamHI (B) (to linearize) and BamHI plus DpnI (B+D) overnight. Digested DNA was resolved and transferred to a GeneScreen membrane (Perkin-Elmer). DpnI-resistant bands were detected after hybridization with a ³²P-labeled puromycin gene probe. Relative densities (RD) of the DpnI-resistant bands were quantified using ImageQuant software (Molecular Dynamics).