FIG. 4.
Reporter gene transfer and protein composition of VLPs generated with single-amino-acid glycoprotein mutants. (A) CAT activities in cells infected with VLPs collected from cells transfected with single-amino-acid glycoprotein mutants. (B) Western blot analysis of the supernatants used for infection in panel A. Blots were analyzed with antibodies recognizing the glycoproteins (GN and GC, upper panel) and the nucleoprotein (N, lower panel). (C) Lysates from wt and mutant glycoprotein-transfected cells were analyzed for the presence of nucleoprotein (N). (D) Single-amino-acid glycoprotein mutants were tested for the ability to transfer the L and S segment-based minigenomes (upper and lower panels, respectively). Asterisks indicate the cellular background.