FIG. 6.
Interaction of the GN glycoprotein cytoplasmic tail with the nucleoprotein. Cells were transfected with L-CAT and two PolI-driven plasmids expressing the full-length vRNA M and S segments, together with the nucleoprotein and polymerase expression plasmid (lane 2) and, in addition, the wt (lane 3) and single-amino-acid-mutant glycoproteins (lanes 4 to 9). Cells were cross-linked and lysed, and proteins were immunoprecipitated with GN monoclonal antibodies. Immunoprecipitated protein complexes were separated by SDS-PAGE under reducing conditions and analyzed by Western blotting using polyclonal antibodies to detect nucleoprotein (N) and glycoprotein (GN). As controls, immunoprecipitates obtained with nontransfected cells (lane 1) and UUK virus-infected cells (lane 10) are shown.