FIG. 5.

NSP4-EGFP expression and thapsigargin treatment suppress cofilin phosphorylation in HEK 293 cells as detected by Western blotting. (A) Induced (Dox +) and uninduced (Dox −) HEK 293/NSP4-EGFP cells were grown in the regular (Calcium +) or low-calcium (Calcium −) medium. Cells were harvested 24 to 48 h postinduction. (B) HEK 293 cells were grown in regular medium and, 8 h prior to harvesting, were treated with 200 nM thapsigargin. Whole-cell lysates were separated by SDS-PAGE, and the indicated proteins were detected by Western blotting with GAPDH as the loading control.