FIG. 6.
Cofilin phosphorylation is also suppressed in rotavirus-infected MA104 cells. (A) Total cofilin and phosphocofilin were detected by Western blotting in whole-cell lysates of rotavirus SA114F-infected (RV infected +) and uninfected (RV infected −) MA104 cells grown in regular (Calcium +) or low-calcium (Calcium −) medium. (B) Rotavirus strain RRV and SA11-infected MA104 cells transfected with RRV and an SA11-specific siRNA (RRV g10 and SA11 g10) to suppress NSP4 expression. A Norwalk virus ORF3 siRNA was used as a negative control.