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. 2007 Jan 31;81(8):3807–3815. doi: 10.1128/JVI.02795-06

FIG. 3.

FIG. 3.

Vif-resistant A3G proteins do not interact with HIV-1 Vif. (A) Analysis of the stability of wild-type (WT) or mutant A3G proteins in the absence or presence of HIV-1 Vif. Proteins were detected by immunoblotting of cell lysates from transfected 293T cells using specific primary antibodies, and Hsp90 was measured as a loading control. (B) Coimmunoprecipitation of HIV-1 Vif with HA-tagged versions of wild-type or mutant A3G. The pCMV4 sample served as the negative control. Whole-cell lysates (top) were analyzed by immunoblotting using antibodies specific for A3G, HIV-1 Vif, or Hsp90 (loading control). The immunoprecipitates (IP) (bottom) were analyzed with antibodies specific for A3G or HIV-1 Vif. The band common to all coprecipitated Vif samples represents antibody light chains.