FIG. 7.

Epitope mapping of MAbs to gB by immunoprecipitation and Western blotting using gB deletion mutants. Mutant proteins were expressed in 293T cells, and their reactivity with MAbs was analyzed. Proteins were either directly probed on a Western blot (C) or were first immunoprecipitated (A, B, and D) with an MAb to gB and then detected by Western blotting with PAb R69. Mutants are labeled at the top of each blot. The MAb used for Western blotting (wb) or immunoprecipitation (ip) is indicated at the bottom of each blot.