FIG. 1.

The HVS ORF73 C terminus associates with MeCP2 but not DEK during in vitro binding assays. (a) Bacterially expressed ORF73C was bound to nickel-agarose beads. (b) The ORF73C-conjugated beads were then incubated with HeLa cell extracts previously transfected with a plasmid expressing an empty FLAG vector (−ve), FLAG-tagged DEK, or FLAG-tagged MeCP2. Proteins attached to the ORF73C-conjugated beads were analyzed by Western blotting using a monoclonal anti-FLAG antibody as a primary antibody. Total-cell extracts representing 5% of total input were run as positive controls (+ve).