FIG. 5.

ORF73 and MeCP2 colocalize on mitotic chromosomes during the metaphase (a) and telophase (b) stages of mitosis. HeLa cells were serum starved, induced to divide by the replenishment of serum, stained with anti-FLAG and Texas red-conjugated antibodies, and visualized by laser scanning confocal microscopy. Images show bright field (i), TO-PRO-3 iodide-stained nucleic acid (ii), ORF73C-GFP (iii), and FLAG-MeCP2 protein (iv) at a magnification of ×400 (a) or ×630 (b). (av and bv) merged GFP and Texas red fluorescent staining, highlighting ORF73 and MeCP2 colocalization on the cellular DNA.