TABLE 2.
Protein or motif | Sequence | HDV assembly (107 GE/ml) | HDV MOI (GE/cell) | HDV infectivity (GE/cell) | HDV specific infectivity |
---|---|---|---|---|---|
wt | 156-PNIASHISSISARTGDPVTNME-177 | 6.9 | 10.4 | 2,690 | 260 |
TLM− | PNIAS------------VTNME | 11.9 | 17.8 | 2,660 | 156 |
S only | 13.4 | 20.1 | 2.84 | 0.14 |
The wt sequence shown is as in shown Fig. 1 and represents that part of the HBV pre-S2 that spans the TLM proposed by Stoeckl et al. (29). The mutation (TLM−) was made using a QuikChange kit (Stratagene). Particles assembled with only HBV S protein were used as a negative control for infectivity. The assembly, infectivity, and specific infectivity of HDV were assayed as described in Table 1 and as previously described (16), and all are based on the average of independent, duplicate, quantitative real-time PCR assays of GE. Based on this and a separate experiment, we consider the range of specific infectivity values for wt and TLM− to be not significantly different.