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. 2007 Jan 31;81(8):4226–4234. doi: 10.1128/JVI.01888-06

FIG. 5.

FIG. 5.

Knockdown of PRMTs by siRNA in different cell lines. A. PRMT6 knockdown 293T, HeLa, and Jurkat cell lines were established using the pSUPER.retro-PRMT6 retroviral vector. Cell lysates (40 μg) were separated on 10% SDS-PAGE. An immunoblot assay with PRMT6-specific antibody was performed; the bands corresponding to PRMT6 protein and the loading control β-actin are indicated. PRMT6 siRNA (6si), PRMT6 mock siRNA (6si m), and empty pSUPER.retro vector (pSUPER) cell lines are shown. B. PRMT1 and PRMT5 knockdown 293T cell lines (1si and 5si, respectively) were established as described for panel A. Immunoblot assays with PRMT-specific antibodies were performed.