FIG. 9.

Titration of LEDGF with RSV IN. Recombinant RSV IN (5 nM) was assembled with a 3.4-kbp donor (0.21 nM) that contained a single wt U3 LTR end for 30 min on ice. LEDGF was added at the final concentration (top) for an additional 10 min. Supercoiled target was added, and the samples were incubated at 37°C for 20 min. The assay conditions were previously described (48), with the final NaCl concentration at 0.2 M. Lanes 1 and 10 were without LEDGF. The percentages of the donor incorporated into FS and CHS products in these two lanes averaged 31% and 3%, respectively. With increasing concentrations of LEDGF (lanes 2 to 9), the percentages of the donor incorporated into FS and CHS products averaged 33% and 2.6%, respectively. Part of the sample in lane 9 was lost.