FIG. 4.

Attachment of HSV-1 hrR3 is not affected by digestion with neuraminidase or Endo H. HSV-1 hrR3 grown in Vero cells was digested with NEB 2-3, V. cholerae neuraminidase, or Endo H (▪) for 4 h at 37°C. For each enzyme condition the virus was mock digested in the same buffer (□). After digestion, attachment was measured by using a CELISA, with the results given as percentages with mock-digested controls defined as 100% for each sample pair. Digestion with A. ureafaciens neuraminidase gave the same results as for V. cholerae neuraminidase (data not shown). The data represent the means and standard deviations of triplicate independent assays. NEB 2-3, S. enterica serovar Typhimurium LT2 neuraminidase; VC, V. cholerae neuraminidase.