FIG. 5.

The absence of gC does not affect the titer reduction seen after neuraminidase digestion. HSV-1 ΔgC2-3 (A) or ΔgC2-3rev (B) was mock digested (□) or digested with the indicated neuraminidase (▪) for 4 h before the titer was determined by plaque assay. Values are reported as percentages, with “mock” defined as 100%. The data represent the means and standard deviations of triplicate independent assays. Digestion with A. ureafaciens neuraminidase gave results identical to those for the V. cholerae enzyme (data not shown). NEB 2-3, S. enterica serovar Typhimurium LT2 neuraminidase; VC, V. cholerae neuraminidase.