TABLE 2.
Amino acid sequences and Z13e1 and 4E10 binding data for a series of truncated MPER peptides
| Peptide | Sequencea | IC50 (nM)b
|
|
|---|---|---|---|
| 4E10 | Z13e1 | ||
| 94-1 | NWFDITNWLWYIKKKK | 15 | >10,000 |
| 132 | SLWNWFDITNWLWYIKKKK | 25 | 110 |
| 160 | DKWASLWNWFDITNWLWYIKKKK | 90 | 15 |
| 179-4 | LLELDKWASLWNWFDITNWLWYIKKKK | 240 | 15 |
| 178-4 | DKWASLWNWFDITNKKKK | >10,000 | 45 |
| 178-3 | WASLWNWFDITNKKKK | >10,000 | 30 |
| 178-2 | SLWNWFDITNKKKK | >10,000 | 550 |
| KGND | KGWNWFDITNWGK-OH | >10,000 | 390 |
a
Boldface indicates residues that correspond to gp41 MPER.
b
The concentration of peptide needed to inhibit by 50% the maximal binding signal that is generated by the MAb and the biotinylated peptide, SLWNWFDITNWLWRRK(biotin)-NH2, in the absence of any competitor peptide, as determined using an ELISA.