TABLE 3.
Neutralization by Z13e1 and 4E10 (Fab and IgG) of pseudotyped HIV-1 by use of a single-round infectivity assay with U87.CD4.CXCR4 (HIV-1MN) or U87.CD4.CCR5 (HIV-1JR-FL and HIV-1JR-CSF) target cells
| Ab or comparison | Concn (nM) or neutralization potency (n-fold) for indicated straina:
|
|||||
|---|---|---|---|---|---|---|
| HIV-1MN
|
HIV-1JR-FL
|
HIV-1JR-CSF
|
||||
| IC50 | IC90 | IC50 | IC80 | IC50 | IC90 | |
| Z13e1 Fab | 3.8 | 145 | 220 | 1,650 | 550 | 3,200 |
| Z13e1 IgG | 1.8 | 47 | 285 | 2,600 | 600 | 4,000 |
| Z13e1 Fab to Z13e1 IgG | 2.1 | 3.1 | 0.8 | 0.6 | 0.9 | 0.8 |
| 4E10 Fab | 1.4 | 20.5 | 35 | 140 | 120 | 650 |
| 4E10 IgG | 0.8 | 7.5 | 45.5 | 235 | 63 | 400 |
| 4E10 Fab to 4E10 IgG | 1.8 | 2.7 | 0.8 | 0.6 | 1.9 | 1.6 |
a
Values in lightface are the Ab concentrations required to diminish virus infectivity by 50, 80, or 90%. For HIV-1MN and HIV-1JR-FL, each value represents the mean of two independent experiments performed in triplicate. For HIV-1JR-CSF, each value represents the mean of triplicate samples (single experiment). Values in boldface are Fab-to-IgG neutralization potency changes (n-fold).