FIG. 3.

Identification and mutational analysis of a consensus PreS-binding motif. (A) Peptide alignment. Highly conserved residues are shaded and in bold. The deduced consensus sequence (pC) is W₁T₂X₃W₄W₅. (B) GST pull-down assays. MBP-PreS1_1-65 was used for the binding assays (lanes 2, 4, and 6). Parallel controls were performed with MBP-PreS1_1-20 (lanes 1, 3, and 5). Lanes 1 and 2, GST-p18; lanes 3 and 4, GST-pC, with the sequence W₁T₂N₃W₄W₅; lanes 5 and 6, W₄W₅→AA mutant of GST-pC. (C) GST pull-down assays with A, F, or S residue substitutions. The substitutions are denoted above each lane.