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. 2007 Jan 31;81(8):4002–4011. doi: 10.1128/JVI.02589-06

FIG. 2.

FIG. 2.

Virus production, RNA packaging efficiency, and virus infectivity of DIS mutants. Virus was produced by transient transfection of 293T cells with wild-type plasmid, pHIVBHO, or the DIS mutant plasmids and pIIINL(AD8)env, encoding HIV-1 Env. (A) Viral particle production as measured by p24 levels. Virus was harvested from the producer cells 48 h posttransfection and assayed for p24 levels by enzyme-linked immunosorbent assay. (B) Efficiency of viral RNA encapsidation. Viral RNA was extracted from a sample of each virus, reverse transcribed, analyzed by real-time PCR using a gag-specific primer-probe set, and normalized to p24 levels. (C) Virion infectivity. Titers of virus were determined on TZM reporter cells containing a Tat-responsive luciferase gene and normalized to p24 levels. All three graphs are plotted as a percentage of the wild-type (WT) levels; shown are the means ± standard errors of results from three independent transfection experiments. Also shown are the DIS sequences and the number of complementary bases within the putative KL complex for each virus assayed.