FIG. 5.

NFX1-123 and PABPC4 synergistically augmented hTERT promoter-regulated luciferase expression through the PAM2 motif in HFKs. (A) By luciferase assay, HFKs expressing HPV16 E6 activated expression from the hTERT promoter, and NFX1-123 augmented that expression only when HPV16 E6 was present. NFX1-123 and PABPC4 coexpressed could further increase expression over NFX1-123 alone, and the PAM2 mutant of NFX1-123 decreased this effect by half. PABPC4 did not increase expression without NFX1-123 present. (B) NFX1-123 augmented activation of HPV16 E6, but NFX1-123 with PAM2 deleted (NFX1-123 ΔPAM2) did not. (C) NFX1-123 and PABPC4 did not activate hTERT promoter-driven expression without HPV16 E6. RLU, relative light units. The error bars indicate 95% confidence intervals.