FIG. 1.

G1 cytoplasmic tails of pathogenic HPS- and HFRS-causing hantaviruses are proteasomally degraded, while the G1 tail of nonpathogenic hantavirus is stable. One microgram of plasmid DNA expressing GAL4-tagged G1 tails of NY-1V, ANDV, HTNV, or PHV was transiently transfected into COS7 cells, and 42 h posttransfection, cells were either treated with 50 μM MG132 for 6 h (+) or left untreated (−). Lysate containing 10 μg of total protein was analyzed on 12% SDS-PAGE gels by immunoblotting with an anti-GAL4 antibody. β-Actin levels were evaluated to demonstrate comparable sample loading.