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The G1 tail of NY-1V, but not that of PHV, directs the chimeric GFP-G1 tail to the proteasome. One microgram of expression plasmid DNA encoding GFP, GFP-NY-1V-G1, or GFP-PHV-G1 was transfected into COS7 cells in the presence (+) or absence (−) of MG132. Fluorescence was detected 48 h posttransfection. (A and B) GFP; (C and D) GFP-PHV G1 tail; (E and F) GFP-NY-1V G1 tail. Each panel shows fluorescence (top) and phase-contrast (bottom) micrographs of the same field.
