FIG. 4.

The degradation signal in the C-terminal 42 residues of the NY-1V G1 tail is independent of lysines at positions 615 and 628. COS7 cells were transfected with 1 μg of plasmid DNA expressing C42 lysine mutants, and 6 h prior to lysis, 50 μM MG132 was added to selected wells (+). Ten micrograms of total protein was analyzed on a 12% SDS-PAGE gel by anti-GAL4 immunoblotting (WB). Blots were reprobed for β-actin to demonstrate comparable sample loading.