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The C-terminal 30 residues of NY-1V G1 tails direct proteasomal degradation. Deletion mutants lacking 5, 14, 30, or 51 residues from the NY-1V G1 tail C terminus were expressed in COS7 cells. Protein expression from MG132-treated (+) and untreated (−) cells was analyzed by Western blotting (WB) using an anti-GAL4 antibody. Blots were reprobed for β-actin to demonstrate comparable sample loading.
