TABLE 1.
Primers used to amplify overlapping PCR fragments
| Primer name | Sequence | 5′ positiona | Type |
|---|---|---|---|
| PW11 | GCGTTAGTATGAGTGTCGTACAGCCTCCAG | 1 | Sense |
| KY78 | CTCGCAAGCACCCTATCAGGCAGT | 288 | Antisense |
| ENO1 | TGGGGATCCCGTATGATACCCGCTGCTTTGA | 8,245 | Sense |
| ENO2 | GGCGGAATTCCTGGTCATAGCCTCCGTGAA | 8,645 | Antisense |
| KS3-2a | GCTTGGGATATGATGATGAACTGGTC | 1,296 | Sense |
| LP2-4a | CGGGGTCGRGCAYGRGACATGCTGTGATAAA | 9,246 | Antisense |
| S1-SW-G2 | ACGGCTGGCCGCTGATGGCATCATACTGGG | 2,951 | Sense |
| AS-SW-G2 | CCCTCGGCGCTGGATAGTACATCTGCGTAG | 3,642 | Antisense |
| S1-SW-G5 | TCATAGTGGTGGCTTCAGTATTTCATAGCCC | 2,806 | Sense |
| AS1-SW-G5 | CCCACCAAGAGTCTTTGTCCTACATTAGGTGTACAT | 3,579 | Antisense |
a
The nucleotide positions are numbered according to the sequence locator tool numbering program from Los Alamos for the strain M67463 subtype 1a.