FIG. 2.

Nuclear translocation of NF-κB-p65 in KSHV-infected cells. Serum-starved HMVEC-d cells and HFF grown in eight-well chamber slides were infected with KSHV (10 DNA copies/cell) for 20 min and 10 min, respectively; washed; fixed; permeabilized; and stained with anti-p65 polyclonal antibody. HMVEC-d cells and HFF were either uninfected (A, B, G, and H) or infected with KSHV (10 DNA copies/cell) (C, D, I, and J) or incubated with 10 μM Bay11-7082, followed by infection with KSHV (E, F, K, and L), and stained for NF-κB-p65. DAPI was used as a nuclear stain and merged with p65 staining.