Skip to main content
. 2007 Feb 7;81(8):3714–3720. doi: 10.1128/JVI.02621-06

FIG. 5.

FIG. 5.

(A) Summary of the effects on the amiloride-sensitive short-circuit current (IscEq-Amil) across the mouse tracheal epithelium of apical application for 1 h of the RSV F protein (2.5 μg/ml), the RSV F protein (2.5 μg/ml) in the presence of the protein kinase C inhibitor BIM-I (100 nmol/liter), and intact RSV (105 PFU/ml) in the presence of BIM-I (100 nmol/liter). (B) Summary of the effects on IscEq-Amil across cultured mouse collecting duct (M1) cell monolayers of application for 1 h of RSV (2 × 105 PFU/ml) and RSV (2 × 105 PFU/ml) in the presence of BIM1 (100 nmol/liter). Also shown are the effects on the inhibition of IscEq by RSV (2 × 105 PFU/ml) of preincubation of the monolayers in PPMP (40 μmol/liter) for 24 h or preincubation in neuraminidase (0.5 U/ml) for 30 min. (C) Summary of the effects on IscEq-Amil across the mouse tracheal epithelium of 1 h of apical exposure to RSV (2 × 105 PFU/ml) or the F protein (2.5 μg/ml) following pretreatment of the apical surface of the epithelium with neuraminidase (Neura) (0.5 U/ml) for 30 min or when apyrase (Apyr) (2 U/ml) or suramin (Sur) (100 μmol/liter) have been included in the apical bathing solution. The data are presented as means ± SEM (with the number of experiments in parentheses). The asterisks indicate statistically significant differences compared to the corresponding controls. con, control.