FIG. 2.

Localization of pSTAT1 is correlated with the localization of P. (A) U373-MG control (upper panel) or P-expressing cell lines were incubated in the absence (middle panel) or presence of LMB (+LMB) at a final concentration of 20 nM for 1.5 h (lower panel). Cells were unstimulated or stimulated with 2,000 U/ml of hIFN-α or IFN-γ for 30 min as indicated, and they were fixed, permeabilized, and then stained with anti-P and anti-pSTAT1 antibodies and DAPI. (B) U373-MG cells were transfected with plasmids expressing P-GFP, P3-GFP (PΔN52-GFP), or PΔN44-GFP. Forty-eight hours after transfection, cells were treated with 2,000 U/ml of hIFN-α or hIFN-γ for 30 min as indicated. Cells were then stained with anti-P and anti-pSTAT1 antibodies and DAPI.