FIG. 2.

Relative efficiencies of conversion reactions, examples of which are shown in Fig. 1. The efficiency for each conversion reaction was determined by quantitation of the amount of input radiolabeled ³⁵S-PrP-sen converted to ³⁵S-PrP-res bands, as described in Materials and Methods. Efficiencies are expressed as the mean percent conversion of ³⁵S-PrP-sen to ³⁵S-PrP-res for 2 to 10 replicate experiments (n), with standard errors of the means (SEM) shown by the error bars. For each PrP-res species, the mean efficiency was normalized (norm x) to the mean conversion efficiency of the homologous (boxed) PrP-sen. All data except those using hamster PrP molecules were previously published (20) and are shown here for comparison.