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. 2007 Feb 7;81(8):4305–4314. doi: 10.1128/JVI.02474-06

FIG. 4.

FIG. 4.

Analysis of PrP-res from sequential passages of CWD inocula in Sg hamsters and Tg (haPrP) mice. (A) Fluorescent immunoblots of SDS-PAGE gels show PK-resistant PrP in CWD primary inocula from individual elk (e), mule deer (md), and white-tailed deer (wd). Equal aliquots of the primary inocula were immunoblotted and analyzed with either 505 antiserum for the left blot or 3F4 Ab for the right blot. The 3F4 Ab detected 263K PrP-res from Sg hamster brain (ha), but it did not detect the PrPCWD in any of the primary inocula. (B and C) Immunoblots of representative examples of first and second serial passages, respectively, into either Sg hamsters or Tg (haPrP) mice, analyzed using 3F4. The sources indicated in panels B and C identify the primary CWD passage inocula. The migration of molecular mass standards, in kilodaltons, is shown to the right of each panel.