TABLE 2.
Serial passage details of individual CWD-positive cervid animal brain homogenates inoculated into Tg (haPrP) mice
| CWD inoculum | Primary passage
|
Second passage
|
Third passage
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Species inoculateda | No. of animals with TSE signs/no. inoculatedb | No. of blot-positive animals/no. tested | Incubation time (dpi) of TSE-positive sample (mean ± SD) | Time (dpi) of blot-positive samples (mean ± SD) | Donor animal inoculumc | Species inoculateda | No. of animals with TSE signs/no. inoculatedb | No. of blot-positive animals/no. tested | Incubation time of animals with TSE signs (mean dpi ± SD) | Donor animal inoculumc | Species inoculateda | No. of animals with TSE signs/no. inoculatedb | Incubation time of animals with TSE signs (mean dpi ± SD) | |
| e | Tg (haPrP) mo | 3/9 | 8/9 | 632 ± 89 | 585 ± 142 | e 1 | Tg (haPrP) mo | 7/7 | 3/3 | 282 ± 39 | e 1a | Tg (haPrP) mo | 4/4 | 203 ± 9 |
| e 1b | Tg (haPrP) mo | 5/5 | 202 ± 7 | |||||||||||
| e 1 | Sg ha | 5/6 | 4/5d | 509 ± 70 | e 1c | Sg ha | 5/5 | 543 ± 39 | ||||||
| e 1d | Sg ha | 5/5 | 526 ± 90 | |||||||||||
| e 2 | Tg (haPrP) mo | 8/8 | 3/3 | 215 ± 9 | e 2a | Tg (haPrP) mo | 4/4 | 210 ± 10 | ||||||
| e 2b | Tg (haPrP) mo | 4/4 | 185 ± 16 | |||||||||||
| e 3 | Tg (haPrP) mo | 7/7 | 3/3 | 212 ± 10 | e 3a | Tg (haPrP) mo | 6/6 | 247 ± 36 | ||||||
| e 3b | Tg (haPrP) mo | 3/3 | 204 ± 9 | |||||||||||
| md | Tg (haPrP) mo | 3/8 | 5/8 | 726 ± 47 | 668 ± 105 | md 1 | Tg (haPrP) mo | 8/8 | 3/3 | 202 ± 8 | md 1a | Tg (haPrP) mo | 4/4 | 213 ± 5 |
| md 1b | Tg (haPrP) mo | 4/4 | 259 ± 33 | |||||||||||
| md 1 | Sg ha | 7/7 | 7/7 | 485 ± 44 | md 1c | Sg ha | 4/4 | 544 ± 110 | ||||||
| md 1d | Sg ha | 2/2 | 540 ± 614 | |||||||||||
| md 2 | Tg (haPrP) mo | 8/8 | 4/4 | 209 ± 28 | md 2a | Tg (haPrP) mo | 3/3 | 228 ± 15 | ||||||
| md 2b | Tg (haPrP) mo | 4/4 | 212 ± 14 | |||||||||||
| wd | Tg (haPrP) mo | 4/9 | 8/9 | 632 ± 73 | 621 ± 80 | wd 1 | Tg (haPrP) mo | 7/7 | 4/4 | 272 ± 62 | wd 1a | Tg (haPrP) mo | 4/4 | 212 ± 32 |
| wd 1b | Tg (haPrP) mo | 4/4 | 237 ± 13 | |||||||||||
| wd 1 | Sg ha | 5/5 | 5/5 | 462 ± 3 | wd 1c | Sg ha | 3/3 | 408 ± 42 | ||||||
| wd 1d | Sg ha | 4/4 | 436 ± 15 | |||||||||||
Abbreviations used are the same as those in Table 1.
For each group, 12 animals were inoculated for the first passage, 8 animals were inoculated for the second passage, and 4 to 6 animals were inoculated for the third passage. Intercurrent deaths, as defined in Table 1, are not included in these data.
The code designates the individual Tg (haPrP) mouse or Sg hamster that was used as a source of inoculum in the passage specified. For example, “e 1” designates an individual TSE-positive Tg (haPrP) mouse that was inoculated with eCWD and was used as the source of inoculum for a second passage; “e 1a” designates an individual Tg (haPrP) mouse from the second passage that had been inoculated from the e 1 Tg (haPrP) mouse.
The blot-negative animal (second passage, e 1 inoculated into Sg hamster) was also negative for neurological signs and likely died due to another undetermined cause.