FIG. 3.

HBV DNA chain termination by ETV. (A) DNAs from isolated HBV nucleocapsids after culture of cells in the presence of [³H]thymidine (TdR) or [³H]ETV (ETV). The migration of molecular size standards and bromphenol blue (BPB) run in parallel is indicated. (B) In vitro RT activity of nucleocapsids isolated from cells cultured with [³H]thymidine or with 100 nM ETV. The incorporation of [³³P]dGTP was plotted based upon the amount of HBV DNA nucleocapsid template used to initiate the reaction. Error bars indicate the standard deviations.