Table 1. The enzymes used for hydrogen production from starch and water, and their reaction mechanisms, sources, and amounts used in the reaction.

E.C.	Enzyme Name	Reaction	Vender	Origin	Unit
2.4.1.1	glycogen phosphorylase	(C6H10O5)n+Pi+H2O→(C6H10O5)n−1+glucose-1-P	Sigma	rabbit muscle	10
5.4.2.2	phosphoglucomutase	G-1-P→G-6-P	Sigma	rabbit muscle	10
1.1.1.49	glucose-6-phosphate dehydrogenase	G-6-P+NADP+→6-phosphogluconate+NADPH	Sigma	S. cerevisiae	1
1.1.1.44	6-phosphogluconic dehydrogenase	6-phosphogluconate+H2O+NADP+→ribulose-5-phosphate+NADPH+CO2	Sigma	S. cerevisiae	1
5.3.1.6	ribose 5-phosphate isomerase	ribulose-5-phosphate→ribose-5-phosphate	Sigma	spinach	1
5.1.3.1	ribulose-5-phosphate 3-epimerase	ribulose-5-phosphate→xylulose-5-phosphate	Sigma	S. cerevisiae	1
2.2.1.1	transketolase	xylulose-5-phosphate+ribose-5-phosphate→sedoheptulose-7-phosphate+glyceraldehyde-3-phosphate	Sigma	E. coli	1
		xylulose-5-phosphate+erythrose-4-phosphate→fructose-6-phosphate+glyceraldehyde-3-phosphate
2.2.1.2	transaldolase	sedoheptulose-7-phosphate+glyceraldehyde-3-phosphate→fructose-6-phosphate+erythrose-4-phosphate	Sigma	S. cerevisiae	1
5.3.1.1	triose-phosphate isomerase	glyceraldehyde 3-phosphate→dihydroxacetone phosphate	Sigma	rabbit muscle	1
4.1.2.13	aldolase	glyceraldehyde 3-phosphate+dihydroxacetone phosphate→fructose-1,6-bisphosphate	Sigma	rabbit muscle	1
3.1.3.11	fructose-1,6-bisphosphate	fructose-1,6-bisphosphate+H2O→fructose-6-phosphate+Pi	[41]	E. coli	1
5.3.1.9	phosphoglucose Isomerase	fructose 6-phosphate→glucose-6-P	Sigma	S. cerevisiae	1
1.12.1.3	P. furiosus hydrogenase I	NADPH+H+→NADP++H2	[22.42]	P. furiosus	∼70