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. 2007 May 16;2(5):e451. doi: 10.1371/journal.pone.0000451

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Narasimhan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A.1. Differential 2D Fluorescence Gel Electrophoresis (DIGE) of 50 µg of proteins from 24 and 66 h fed salivary glands. Highlighted are proteins at positions 1 and 2 preferentially expressed at 66 h, while proteins at positions 3 and 4 are differentially expressed in 24 h salivary glands. A.2. Histogram of the global protein profiles plotted as a log ratio of spot intensity of Cy5/Cy3 (66 h/24 h) shows a set of proteins that are greater than 3 fold differentially expressed in 24 h (indicated by red circles) and 66 h (indicated by blue circles). Proteins less than 3 fold differentially expressed (indicated by green circles) are represented within the bell curve. B. A western blot of 5 µg of proteins isolated from 24 and 66 h fed salivary gland extracts probed with nymph-immune rabbit serum shows greater reactivity with proteins from the 24 h extracts than from the 66 h fed extracts.