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. 2007 May 16;2(5):e451. doi: 10.1371/journal.pone.0000451

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Narasimhan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Chromatogram of the profiles of protein extracts from 24 h (shown in red) 66 h (shown in black) fed salivary glands fractionated on a C4 hydrophobic column shows differences in the protein composition of the two populations. Fraction numbers are indicated next to prominent protein peaks. B. A dot-blot analysis of the HPLC fractions using tick-immune rabbit serum demonstrates differential reactivity with 24 and 66 h protein fractions. Numbers next to the cross-reacting spots correspond to HPLC protein peaks.