Table 1. Expression profiles of Ixodes scapularis salivary genes at 24 and 66 h of feeding.

Gene accession number	Putative function/homology	Ratio of 24 h/66 h pixel intensities	P value
AF483716	Protease inhibitor	−10	6e-5
AF483714	Putative protease inhibitor	+4	1e-2
AF483727	Putative serpin	+10	1e-2
AF483726	Protease inhibitor	+10	1e-2
AF483689	Secreted protease inhibitor	−3	1e-2
AF483712	Salp10-like	+10	6e-5
AF278575	Salp10	−20	1e-2
AF483719	Salp13 -like	1	6e-5
AF483720	-Salp13-like	−2	9e-3
AF209912	Salp13	−100	1e-2
AF483728	Secreted carboxypeptidase	−10	6e-5
AF483731	Secreted metalloprotease	1	6e-5
AF483740	Secreted 10.2 kDa	−3	6e-5
AF483688	Secreted convertase protease	−5	1e-3
AF483729	Transmembrane serine protease	−10	1e-3
AAY333959	Thoredoxin peroxidase	−20	1e-3
AF483690	Secreted protease	+7	5e-4
AF483743	Secreted lipocalin	−6	6e-5
AF483722	Lipocalin-like	1	6e-5
AF209920	Histamine binding protein	−20	1e-2
AF483718	Histamine-binding protein	+10	1e-2
AF209918	Salp25B-Histamine binding	−10	1e-2
AF483721	Salp25A-like	1	1e-1
AF209922	Salp25A-Histamine binding	−8	5e-4
AF483734	5.3 Kda protein/defense	1	6e-5
AF483737	Secreted protein	−10	6e-5
CD052513	Similar to Virilizer	+3	6e-5
AF209916	Salp17	−20	6e-5
AF483659	Salp14-like	1	1e-4
AF483662	Salp14-like	1	1e-4
AF483665	Salp14-like	+2	5e-4
AF483660	Salp14-like	+2	1e-2
AF483663	Salp14-like	1	1e-2
AAY66642	Secreted protein	+10	1e-2
AF209921	Salp14- anticoagulant	−4	1e-2
AF483663	Salp14-like	1	1e-2
AF483703	Secreted protein	−10	2e-4
AF483681	Secreted protein	−2	5e-4
AF483686	Secreted protein	−100	1e-2

Results presented as a ratio of the median of normalized intensities from Cy5 (24 h salivary gland) to the median of normalized intensities from the Cy3 (66 h salivary gland). P values calculated from the paired signed-rank test are shown.