Table 3.

Distribution of ESTs and SAGE Tags in Five Alternatively Polyadenylated Genes^a

Gene	Site no.	No. of ESTs	SAGE tag	No. of SAGE tags	Distance from site 1
PDXK	1	35	AGTGTCCGGC	186
Pyridoxal kinase	2	9	TAAACAGTTG	16	2.8 kb
	3	23	TGGATGTTTG	12	6.2 kb
CSTB	1	130	TTGGTGCTTT	27
Cystatin B	2	4	CAAAACAGAA	22b	1.4 kb
AGPAT3	1	15	GCGGAGCTGG	12
1-acylglycerol-3-phosphate	2	14	GGGTCTCCTG	99	0.75 kb
O-acyltransferase 3	3	15	AACACTCGCC	52	1.9 kb
	4	13	ACAGCCACTG	62	2.5 kb
TMEM1	1	5	TAATTGTAGC	12
Transmembrane protein 1	2	5	GGATGTCCTA	14	1.5 kb
	3	7	GTTTACTATG	12	1.8 kb
SMT3H1	1	11	CCGACCACAA	121
SMT3 (suppressor of mlf two 3,	2	11	TAACCTCGGG	57	0.85 kb
yeast) homolog	3	85	TTCTTCTCGT	263	1.1 kb

^aFor five representative genes from Table 2, the number of EST contributing to the definition of the most prominent polyadenylation sites was counted, NIaIII SAGE tags immediately upstream of the polyadenylation sites were deduced, and the cumulative representation of the tags in SAGE libraries was determined for each of the major polyadenylation sites. The sites are listed in the 5′ to 3′ direction relative to the transcript, including the distance from the 5′ most site for those lying downstream. 

^bThis SAGE tag is not unique to the CSTB gene; it also occurs in a transcript from gene AF5Q31 (ALL1 fused gene from 5q31). 

SAGE, serial analyses of gene expression.