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. Author manuscript; available in PMC: 2007 May 8.
Published in final edited form as: Hum Mol Genet. 2006 Mar 2;15(7):1087–1098. doi: 10.1093/hmg/ddl023

Figure 2.

Figure 2

Growth factors stimulate the novel NF1/Ras-dependent AC pathway. (A) AC activity was significantly increased by treatment of larval brains with 2 mm EGF (n = 18). This stimulation was abolished in EGFR mutants, Egfrt1, and heterozygotes, Df(2R)Egfr18/CyO; in Nf1 null mutants, Nf1P1 and Nf1P2; and in Ras heterozygotes, Rase2F/TM3 and Rase1b/TM3 (n = 4). (B) Stimulation of AC by 2 mm TGFα was similarly abolished in the Egfrt1 mutant, Nf1 mutants and Ras heterozygotes (n = 4). Stimulation of AC by 2 mm EGF (C) or TGFα (D) is not affected in a hypomorphic Gαs mutant, GsαB19, whereas stimulation by 20 mm GTPγS is perturbed (n = 3). (E) There was no stimulation of AC by 2 mm insulin (n = 3). (A–E) Values are mean ± SEM (**P < 0.01; ***P < 0.005).