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Western blot quantification of EphB4 protein demonstrating the efficiency of EphB4 siRNA in transfected cells. The membrane was reprobed for β-actin to check for equal loading. NT, nontransfected cells; control siRNA, luciferase siRNA; transfection reagent, Dharmafect2. n = 3. ***P < 0.001 versus nontransfected cells.
