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. 2007 May 17;117(6):1527–1537. doi: 10.1172/JCI28338

Figure 7. EphB4 activation mediates EPC adhesion through PSGL-1.

Figure 7

(A and B) PSGL-1 expression in EPCs. EPCs were stimulated with 3 μg/ml of either ephrin-B2–Fc, EphB4-Fc, or CD6-Fc and then processed for FACS analysis of PSGL-1 expression. (A) FACS profiles of nontransfected (top panel) and EphB4 siRNA–transfected (bottom panel) EPCs. (B) Expression of PSGL-1 in nontransfected (top panel) and EphB4 siRNA–transfected (bottom panel) EPCs. n = 3. **P < 0.01 versus nonstimulated EPCs. (C) Effect of PSGL-1 siRNA on PSGL-1 protein expression. Control siRNA– and PSGL-1 siRNA–transfected EPCs were stimulated with 3 μg/ml ephrin-B2–Fc or left unstimulated and then processed for immunocytochemistry with an anti–PSGL-1 antibody as described in Methods. PSGL-1–positive staining appears in red. Scale bar: 20 μm. (D) Effect of PSGL-1 siRNA on EPC adhesion to IL-1β prestimulated HUVEC monolayer. Adhesion was quantified by measuring OD at 570 nm. n = 3. *P < 0.05 versus nonstimulated EPCs transfected with control siRNA.