Figure 4. Aggrecan degradation in vitro.

(A) Aggrecan release from wild-type (white bars) and Jaffa (gray bars) cartilage cultured with or without 10 ng/ml IL-1α (n = 8–10 per treatment). Results are mean ± SEM. The dotted line shows the level of baseline release. ***P < 0.001. (B) Cartilage extracts from wild-type and Jaffa mice with or without IL-1α treatment were analyzed by Western blot. Membranes were immunolocalized for VTEGE³⁷³ neoepitope.