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The 1H 15N HSQC NMR spectrum of the wild type protein (black) overlaid with the spectrum of the C186P·A220P mutant (red). Spectra were recorded at 800 MHz in buffer containing 25 mM Tris , 50 mM NaCl, 50 mM arginine, 50 mM glutamic acid, 1 mM DTT, 5mM CHAPS and 0.5 mM EDTA in 90% H2O 10% D2O at pH 7.5 and 15 °C.
