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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Anal Biochem. 2006 Dec 5;361(1):47–54. doi: 10.1016/j.ab.2006.11.021

Figure 2.

Figure 2

Biological activity elicited by labeled EGF. EGF from four sources (denoted as A,B,C,D) was conjugated to IRDye® 800CW and purified by HPLC. A431 cells at 90% confluence in 96-well plates were stimulated with the indicated concentrations of the respective agents or with the maximally active FPLC-purified conjugates corresponding to sources A and B. The plate was scanned to quantify binding of the IRDye® 800CW EGF targeting agents (A). The extent of ERK phosphorylation stimulated in each condition was assessed in the same plate by the in-cell western assay using antibodies to total ERK and p-ERK (B). Mean ± SD for three replicate wells is plotted.